Application

Research CategorySignaling

Anti-OTUD3, clone 12A6.1, Cat. No. MABS1819, is a mouse monoclonal antibody that detects OTUD3 in human cells and has been tested for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

OTU domain-containing protein 3 (UniProt: Q5T2D3; also known as EC: 3.4.19.12, OTUD3) is encoded by the OTUD3 (also known as KIAA0459) gene (Gene ID: 23252) in human. OTUD3 is a cytoplasmic deubiquitinating enzyme that hydrolyzes Lys-6- and Lys-11-linked polyubiquitin and is reported to hydrolyze heterotypic (mixed and branched) and homotypic chains. It has an OUT domain (aa 65-189) and a UBA-like domain (aa 230-270). Its specificity is given by the S1′ ubiquitin-binding site within the OTU domain composed of the Cys-, His- and Variable-loops. The UBA-like domain has no effect on ubiquitin hydrolysis. It displays a thiol-dependent ubiquitin-specific protease activity. It is reported to regulate PTEN stability and suppresses tumorigenesis. OTUD3 transgenic mice exhibit higher levels of the PTEN and are shown to be less prone to tumorigenesis. OTUD3 is believed to regulate PTEN in a manner, which is dependent on its DUB activity. The catalytically inactive mutant C76A does not upregulate PTEN and may even have a downregulating effect. It can remove Lys-6, Lys-11, Lys-27, and Lys-48 types of ubiquitin chain on PTEN. (Ref.: Yuan, L., et al (2015). Nat. Cell Biol. 17(9); 1169-1181).

Immunogen

GST/His-tagged recombinant fragment corresponding to 115 amino acids from the C-terminal half of human OTU domain-containing protein 3.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in human cerebral cortex tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected OTUD3 in 10 µg of human cerebral cortex tissue lysate.

Specificity

Clone 12A6.1 detects OTU domain-containing protein 3 in human cells. It iargets an epitope within 115 amino acids from the C-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~48 kDa observed; 45.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).